Textbook of Clinical Biochemistry and Diagnostic Technique is a comprehensive textbook designed for undergraduate, postgraduate, and allied health science students. It provides a clear understanding of the principles of clinical biochemistry, laboratory diagnostic techniques, and the biochemical basis of health and disease. The book covers fundamental concepts, analytical methods, quality assurance, instrumentation, biomolecules, enzymes, hormones, organ function tests, electrolyte and acid–base balance, clinical enzymology, immunodiagnostics, molecular diagnostics, and recent advances in laboratory medicine. Emphasizing both theoretical knowledge and practical applications, it integrates case-based learning, diagnostic interpretation, and modern laboratory practices, making it a valuable resource for students, educators, researchers, and clinical laboratory professionals.